Fig. 7. Effect of housing temperature on fasting plasma concentrations of lipids, ketone bodies, hepatic inflammatory markers, gluco- and appetite-regulating hormones, and glucose tolerance after an oral glucose.

Plasma concentrations of TG, 3-HB, cholesterol, HDL, ALT, AST, FFA, glycerol, leptin, insulin, C-peptide, glucagon, and FGF21 are shown in adult DIO male mice (a–o) after 33 days of housing at the indicated temperatures. Mice had been fasted 2–3 h prior to collection of blood. The oral glucose tolerance test is an exception to this as it was performed two days before study end on mice that had been fasted 5–6 h and housed 31 days at the respective temperatures Mice were challenged with 2 g/kg body weight. Area under the curve data (o) are shown as incremental data (iAUCs). Data are presented as means ± SEM. Dots indicate individual samples. *P < 0.05, **P < 0.01, **P < 0.001, ****P < 0.0001, n = 7.