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. 2022 Sep 10;13(9):782. doi: 10.1038/s41419-022-05226-5

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© The Author(s) 2022, corrected publication 2022

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Fig. 4 — A FISH analyses of circGMCL1 and miR-124-3p in NCM460 cells. B Luciferase reporter analysis showing the interaction between miR-124-3p and circGMCL1 in NCM460 cells. C Relative expression of circGMCL1 and GMCL1 in NCM460 cells transfected with three siRNAs as determined by qRT-PCR tests. Relative circGMCL1 (D) and miR-124-3p (E) expression in NCM460 cells transfected with control, oe-, si-circGMCL1 by qRT-PCR. F Nucleic acid electrophoresis and qRT-PCR analyses of circGMCL1 and miR-124-3p expression based on the Ago2 RIP assay. G Enrichment analysis of circGMCL1 and miR-124-3p after RNA pull down assay with circGMCL1 or miR-124-3p probe as evaluated by qRT-PCR assay. H Luciferase reporter analysis showing the interaction between miR-124-3p and ANXA7 in NCM460 cells. I Relative ANXA7 expression in NCM460 cells transfected with oe-, si-circGMCL1, miR-124-3p mimic, and inhibitor. FISH fluorescence in situ hybridization, WT wild-type; MUT, mutant; qRT-PCR, quantitative real time-polymerase chain reaction; RIP, RNA immunoprecipitation; ANXA7, annexin 7. Data are expressed as mean ± SD. **P < 0.01, ***P < 0.001 by Student’s t or one-way ANOVA test.