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. 2022 Sep 10;13(9):782. doi: 10.1038/s41419-022-05226-5

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© The Author(s) 2022, corrected publication 2022

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Fig. 5 — A Results of western blotting showing protein levels of ANXA7, LC3B, NLRP3, ASC, and GSDMD. B Quantitative results of ANXA7 expression in western blotting test. Immunofluorescence (C) and quantitative analysis (D) of LC3B expression by western blotting in NCM460 cells. E Representative TEM images of the autophagosome in NCM460 cells transfected with oe-circcGMCL1. F Autophagy flux based on mCherry-GFP-LC3B retrovirus generation in NCM460 cells transfected with control, oe- and si-circGMCL1. G Immunofluorescence of NLRP3 and caspase-1 in NCM460 cells. Quantitative analysis of ASC H and NLRP3 I following western blotting test in NCM460 cells. GSDMD immunofluorescence (J) and TUNEL (K) assays in NCM460 cells. Representative pyroptosis SEM image (L). Proinflammatory cytokines (IL-1β and IL-18) in NCM460 cells M. QRT-PCR quantitative real time-polymerase chain reaction, ANXA7 annexin 7, TEM transmission electron microscope. Data are presented as mean ± SD. **P < 0.01, ***P < 0.001 by one-way ANOVA test.