Table 2.
Primers sequences.
| Name | Sequence (5′-3′) |
|---|---|
| Full-length sequencce of circGMCL1 | GTGCCTTGAATGGCTTCTAAACAATTTGATGACTCACCAGAATGTTGAACT |
| TTTTAAAGAACTCAGTATAAATGTCATGAAACAGCTCATTGGTTCATCTAAC | |
| TTATTTGTGATGCAAGTGGAGATGGATATATACACTGCTCTAAAAAAGTGGA | |
| TGTTCCTTCAACTTGTGCCTTCTTGGAATGGATCTTTAAAACAGCTTTTGAC | |
| AGAAACAGATGTCTGGTTTTCTAAACAGAGGAAAGATTTTGAAGGTATGGC | |
| CTTTCTTGAAACTGAACAAGGAAAACCATTTGTGTCAGTATTCAGACATTT | |
| AAGGTTACAATATATTATCAGTGATCTGGCTTCTGCAAGAATTATTGAACAA | |
| GATGCTGTAGTACCTTCAGAATGGCTCTCTTCTGTGTATAAACAGCAGTGGT | |
| TTGCTATGCTGCGGGCAGAACAGGACAGTGAGGTGGG | |
| Human circGMCL1 | |
| Forward | AGTACCTTCAGAATGGCTCTCT |
| Reverse | TTTAGAAGCCATTCAAGGCACC |
| Human miR-124-3p | |
| Forward | CATAAGGCACGCGGTGAATG |
| Reverse | GTCGTATCCAGTGCAGGGTC |
| RT Primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGA |
| TACGACTTGGCA | |
| miR-124-3p mimic | UAAGGCACGCGGUGAAUGCC |
| miR-124-3p inhibitor | GGCAUUCACCGCGUGCCUUA |
| Human ANXA7 | |
| Forward | ACGTTTACTTGTGTCCATGTGC |
| Reverse | AGATTCATCGGTCCCTAGTCT |
| Human GMCL1 | |
| Forward | CCACGGATTCTGTTACTGTGC |
| Reverse | TCCTCATCCTCGTCCGTCT |
| Human GAPDH | |
| Forward | TCCTGGGCTACACTGAGCAC |
| Reverse | CTGTTGCTGTAGCCAAATTCGTTG |
| Mouse circGMCL1 | |
| Forward | ATTCTCTGGTACCTTCAGTGGA |
| Reverse | ACATCTGTTTCTGTCAAAAGCTG |
| Mouse miR-124-3p | |
| Forward | CCTAAGGCACGCGGTGAATG |
| Reverse | GTCGTATCCAGTGCAGGGTC |
| RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCA |
| CTGGATACGACGGCATT | |
| Mouse ANXA7 | |
| Forward | CTTCGATGACTCAGGGCACC |
| Reverse | ACGGTTAGACACAACATCCACA |
| Mouse GMCL1 | |
| Forward | CAGGTCGCATTTGGATCACT |
| Reverse | CGCACTGCTGTATCAAACCAT |
| Mouse GAPDH | |
| Forward | GTCAAGGCCGAGAATGGGAA |
| Reverse | CTCGTGGTTCACACCCATCA |
| U6 | |
| Forward | ATTGGAACGATACAGAGAAGATT |
| Reverse | GGAACGCTTCACGAATTTG |
| Si-circGMCL1-1 | Target: GTGAGGTGGGGTGCCTTGAAT |
| Sense | UCAAGGCACCCCACCUCAC |
| Antisense | GAGGUGGGGUGCCUUGAAU |
| Si-circGMCL1-2 | Target:GTGGGGTGCCTTGAATGGCTT |
| Sense | GTGGGGTGCCTTGAATGGCTT |
| Antisense | GGGGUGCCUUGAAUGGCUU |
| Si-circGMCL1-3 | Target: GCCTTGAATGGCTTCTAAACAAT |
| Sense | UGUUUAGAAGCCAUUCAAGGC |
| Antisense | CUUGAAUGGCUUCUAAACAAU |
| Oe-circGMCL1 | - |
| Sense | GGAATTC TGAGGTGGGGTGCCTTGAAT |
| Antisense | CCCAAGCTT AGATCCATTCCAAGAAGGCACAA |
| circGMCL1 FISH probe | 5′-FAM-AGCCATTCAAGGCACCCCACCTCACTGTCC-3′ |
| miR-124-3p FISH probe | 5′-CY3-TTGGCATTCACCGCGTGCCTTA-3′ |
qRT-PCR quantitative real time-polymerase chain reaction, ANXA7 Annexin 7.