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. 2022 Sep 10;13(9):783. doi: 10.1038/s41419-022-05224-7

Fig. 1. Elevating P130cas enhances the proliferation of NSCLC in vitro and in vivo.

Fig. 1

A Stable up- or down-regulation of P130cas expression by two different shRNAs were confirmed by western blotting in A549 cells and H1299 cells, respectively. Colony formation assay (B), MTT assay (C), and cell cycle analysis (D) using the A549-NC and -P130cas clones, and H1299-NC and –shP130cas#1 and #2 clones. Quantification data are expressed as Mean ± SD of three independent experiments (t-test, two-sided, *P < 0.05, **P < 0.01, ***P < 0.001). E Western blotting assay detected the expression of cell cycle-related proteins in the A549-NC and -P130cas clones and H1299-NC and –shP130cas #1 and #2 clones (three biological replicates). F, G Representative examples of explanted tumors in the LLC-P130cas and LLC-NC study group. Quantification data are expressed as Mean ± SD of three independent experiments (t-test, two-sided, *P < 0.05, **P < 0.01, ***P < 0.001). For Western blot experiments, the samples derive from the same experiment and the gels/blots were processed in parallel.