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. 2022 Sep 10;5:947. doi: 10.1038/s42003-022-03894-9

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Target sequence located in ITS2 region of Crocus sativus, Cs_target1 and its crRNA, Cs_crRNA. The red sequence is PAM and purple sequence is protospacer in Cs_target1; The gray sequence is the universal sequence of crRNA for LbaCas12a and purple sequence is spacer in Cs_crRNA. b Density of Targets’ copy numbers ranging from 1 to 300 in Cr. sativus. Cs_target1 has 201 copies in the genome (a).