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. 2022 Sep 10;5:947. doi: 10.1038/s42003-022-03894-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Selection of target sequences. The red lines represented Cs_target1 and its copies and white lines represented the sequences within three base mismatches compared to Cs_target1. b Results of the identification of Cr. sativus with GAGE. The plant materials in the circle, square, triangle, and inverse triangle are the stigma of saffron, the flower of safflower, the stamen of lotus, and the style and stigma of corn, respectively. The DNA substrates of each group were as follows: Cs: ITS2 fragments of Cr. sativus; Ct: ITS2 fragments of Ca. tinctorius; Nn: ITS2 fragments of N. nucifera; Zm: ITS2 fragments of Z. mays and CK (negative control): nuclease-free water. All plots data represent means +/− standard deviation (SD) from three independent replicates.