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. 2022 Sep 10;5:947. doi: 10.1038/s42003-022-03894-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Statistics of Targets in different classification derived from the four genomes. Genome: Targets located in the whole genome; Unannotated regions: Targets located in the unannotated regions of genome; Coding genes: Targets located in coding genes; CDS: Targets located in CDS. b Result of employing GAGE in plants from different classes. The DNA substrates and crRNA of every group were as follow: Rc: ITS2 fragments of R. communis + Rc_crRNA; Si: ITS2 fragments of Set. italica + Si_crRNA; Gb: ITS2 fragments of G. biloba + Gb_crRNA; As: ITS2 fragments of A. spinulosa + As_crRNA; St: ITS2 fragments of Sel. tamariscina + St_crRNA and CK (negative control): nuclease-free water + Cs_crRNA. All plots data represent means +/− standard deviation (SD) from three independent replicates.