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. 2022 Sep 10;5:949. doi: 10.1038/s42003-022-03904-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Quantification of intermediates and final product strictosidine by UPLC/MS analysis. b Absence of MLPL reduces strictosidine production >60 fold while supplementation of GPPS improves yield ~5 fold. c the total ion chromatogram of leaf tissue infiltrated with the entire pathway to strictosidine (including DXS, GPPS, and MLPL). The peak at 4.09 min retention time in the total ion chromatogram (TIC) and extracted ion chromatogram (EIC) at 531.2336 m/z matches a strictosidine standard. N.D., not detected; 7-DLH, 7-deoxyloganic acid hydroxylase; LAMT, loganic acid O-methyltransferase; SLS, secologanin synthase; TDC, tryptophan decarboxylase; STR, strictosidine synthase. Values and error bars represent the mean and the standard error of n = 3 or n = 6 biological replicates (independent leaf samples).