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. Author manuscript; available in PMC: 2023 Jan 13.
Published in final edited form as: Nature. 2022 Jul 13;607(7919):610–616. doi: 10.1038/s41586-022-04939-z

Extended Data Figure 2: Modular Architecture of GATOR2.

Extended Data Figure 2:

(a) Two orthogonal views of the molecular model of GATOR2.

(b), (c), (d) Molecular models (top) and corresponding domain schematics (bottom) of three distinct building blocks of GATOR2: MIOS-SEH1L (b), WDR24-SEH1L (c), and WDR59-SEC13 (d). Domains not resolved in our cryo-EM structure are drawn with increased transparency for reference.

(e) Each core GATOR2 subunit (MIOS, WDR24, WDR59) associates with either SEH1L or SEC13. Anti-FLAG immunoprecipitates were prepared from HEK293T cells that transiently expressed the indicated cDNAs and were analyzed by immunoblotting for the indicated proteins. HA, hemagglutinin.

Data in (e) are representative of two independent experiments. For gel source data, see Supplementary Figure 1.