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. Author manuscript; available in PMC: 2023 Jan 13.
Published in final edited form as: Nature. 2022 Jul 13;607(7919):610–616. doi: 10.1038/s41586-022-04939-z

Extended Data Figure 4: GATOR2 CTDs contain RING domains.

Extended Data Figure 4:

(a) Sequence alignment of the homologous C-terminal regions of MIOS, WDR24, and WDR59. Zinc-coordinating residues are highlighted in yellow. Zinc-coordinating residues in the ZnF are indicated by red dots. Zinc-coordinating residues in the RING domain are indicated with blue dots.

(b) Side-by-side comparison of the structurally-aligned MIOS, WDR24, and WDR59 CTDs.

(c) Structural comparison of the MIOS, WDR24, and WDR59 RING domains with the E3 ligase RNF4 RING domain (PDB ID: 4AP4)17.

(d), (e), and (f) Detailed views of the experimental map and the corresponding molecular model surrounding the zinc coordination sites within the MIOS CTD ZnF (d) and RING domain, (e) and (f).

(g) Schematic of zinc ion coordination in the MIOS CTD. The MIOS CTD coordinates a total of four zinc ions with 15 amino-acid side chains — similarly to the CTDs in WDR24 and WDR59.

(h) Cartoon representation of the zinc ion coordination in a canonical RING domain associated with E3 ubiquitin ligase activity. The RING domain coordinates two zinc ions with eight side chains.