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. 2022 Sep 7;16:2981–2993. doi: 10.2147/DDDT.S374328

Figure 6.

Figure 6

Ferroptosis or Nrf2 inhibitors diminished the cytotoxic effect of LA in BC cells. MDA-MB-231 cells were cultured in 96-well plates. Ferrostain-1 (2.5 μM) or liproxstatin-1 (2 μM) were used as ferroptosis inhibitors, and ML385 (10 μM) was selected as Nrf2 inhibitors. All inhibitors were incubated with LA (40 μM) for 24 hours. The viability of MDA-MB-231 cells was tested by MTT assay, and the expressions of SLC7A11, Nrf2 and HO-1 were detected using immunofluorescence staining. (A) The viability of MDA-MB-231 cells was determined in different groups. (B-D) The expressions of SLC7A11, Nrf2 and HO-1 were analyzed using immunofluorescence staining. Bar=200 μm. (E-G) Statistical analysis of the fluorescence intensity of SLC7A11, Nrf2 and HO-1 in MDA-MB-231 cells. The experiments were repeated at least three times with 3 replicates, and data are expressed as the mean ± SD. **P <0.01 compared with the control group, #P < 0.05, ##P < 0.01 compared with the LA-treated group.