Fig. 1.

Phase contrast and scanning electron microscopy (SEM) and immunocytochemical analyses of PK-8 cells under 2D and 3D culture conditions.

Representative phase contrast and SEM images of PK-8 cells in 2D (upper panels) and 3D (lower panel) cultures. E-cadherin and vimentin immunostaining of cell blocks from adherent PK-8 cells (upper panels) or PK-8 spheres (lower panel). Arrows indicate E-cadherin-positive cells. Scale bar in the phase contrast image of 2D culture: 200 μm, 3D culture:100 μm; SEM images in 2D culture: 20 μm, 3D culture: 50 μm; immunocytochemistry: 50 μm.