REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-HA-Biotin, High Affinity (3F10)	Roche	Cat#12158167001; RRID:AB_390915
Horseradish peroxidase conjugated Goat anti Rat IgG antibody	Bio-Rad	Cat#5201–2504
Anri-Lysophospholipase 1 antibody [EPR3667]	Abcam	Cat#ab91606; RRID:AB_10565192
Anti-APEH antibody	Atlas antibodies	Cat#HPA029700; RRID:AB_10599278
Anti-Biotin antibody produced in goat	Sigma	Cat#B3640; RRID:AB_258552
Bacterial and virus strains
XL10-Gold Ultracompetent cells	Aligent	Cat#200315
Biological samples
Anonymized human blood	United Kingdom National Health System Blood and Transplant Special Health Authority	N/A
Chemicals, peptides, and recombinant proteins
TMT Mass Tagging reagents	Thermo Fisher Scientific	Cat#90308/90309 Lot#RH239931/239932
ActivX™ TAMRA-FP Serine Hydrolase Probe	Thermo Fisher Scientific	Cat#88318
ActivX™ Azido-FP Serine Hydrolase Probe	Thermo Fisher Scientific	Cat#88316
Compound 2 (C2)	Kind gift of Dr Simon Osbourne, LifeArc, SBC Open Innovation Campus, Stevenage	n/a
Biotin-alkyne	Jena Bioscience	Cat#CLK-1134
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)	Aldrich	Cat#C4706
tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA)	Sigma	Cat#678937
Trypsin Gold	Promega	Cat#V5280
SalI restriction endonuclease	New England Bioscience	Cat#R0138
BglII restriction endonuclease	New England Bioscience	Cat#R0144
Phusion® High-Fidelity DNA Polymerase	New England Bioscience	Cat#M0530
Geneticin selective Antibiotic G418 Sulphate	Gibco	Cat#11811023
ML348	Sigma	Cat#SML1901
ML349	Sigma	Cat#SML1918
AA74-1	Sigma	Cat#CAY17507
P11	Cayman Chemical	Cat#CAY17507
Pyridostigmine bromide (PyBr)	Sigma	Cat#P9797
Tri-o-cresyl phosphate (TOCP)	Supelco	Cat#51885
JW480	Sigma	Cat#SML17507
TBV-3166	Sigma	Cat#SML1694
132A RNA dye	Kind gift of Prof YT Chang, National University of Singapore	n/a
Pierce NeutrAvidin Agarose	Thermo Fisher Scientific	Cat#29200
Pierce Control Agarose resin	Thermo Fisher Scientific	Cat#26150
RMPI-1640 based media supplemented with 0.292 g/L L-glutamine, 25 μg/ml gentamycin and 5% (w/v) Albumax II	Gibco	Cat#04191762A
Percoll	GE Healthcare Life Science	Cat#17-0891-01
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#23225
Empore Octadecyl C18 47mm Extraction Disks 2215	Supleco	Cat#66883-U
pH reversed-phase peptide fractionation kit	Pierce	Cat#84868
WR99210	Jacobus Pharmaceuticals	n/a
Infusion HD Cloning Kit	ClonTech	Cat#102518
DNeasy Blood and Tissue Kit	Qiagen	Cat#69504
QIAquick PCR purification kit	Qiagen	Cat#28104
SYBR Safe	Thermo Fisher Scientific	Cat#S33102
QIAprep Midi Kit	Qiagen	Cat#12943
P3 Primary cell 4-D Nucleofector X kit	Lonza	Cat#V4XP-3034
Deposited data
Life cycle proteomics data	ProteomeXchange Consortium via PRIDE. http://www.ebi.ac.uk/pride	Project accession PRIDE: PXD035891
Experimental models: Cell lines
P. falciparum 3D7	Professor Mike Blackman, The Francis Crick Instiutue	n/a
P. falciparum B11	Professor Mike Blackman, The Francis Crick Instiutue (Perrin et al., 2018)	n/a
P. falciparum Psta1-cMUT	This paper	n/a
P. falciparum Psta2-cMUT	This paper	n/a
P. falciparum abH112-cMUT	This paper	n/a
P. falciparum abH114-cMUT	This paper	n/a
Oligonucleotides
Primers for Pf cMUT generation	See Table S2 for list of primers and sequences
Recombinant DNA
pT2A-Psta1-cMUT	This paper (adapted from pT2A_FIKK10_cKO gift of Dr M. Treeck, The Francis Crick Institute)	n/a
pT2A-Psta2-cMUT	This paper (adapted from pT2A_FIKK10_cKO gift of Dr M. Treeck, The Francis Crick Institute)	n/a
pT2A-ab112-cMUT	This paper (adapted from pT2A_FIKK10_cKO gift of Dr M. Treeck, The Francis Crick Institute)	n/a
pT2A-ab114-cMUT	This paper (adapted from pT2A_FIKK10_cKO gift of Dr M. Treeck, The Francis Crick Institute)	n/a
Psta1 synthetic DNA for the generation of pT2A-Psta1-cMUT	GENEWIZ	n/a
Psta2 synthetic DNA for the generation of pT2A-Psta2-cMUT	GENEWIZ	n/a
abH112 synthetic DNA for the generation of pT2A-abH112-cMUT	GENEWIZ	n/a
abH114 synthetic DNA for the generation of pT2A-abH112-cMUT	GENEWIZ	n/a
Software and algorithms
MaxQuant v1.6.0.1	Max-Planck-Institute of Biochemistry	https://maxquant.org
Perseus v1.5.6.0	Max-Planck-Institute of Biochemistry	https://maxquant.org
FACSDiva software v8.0.1	BD biosciences	https://www.bdbiosciences.com/en-ca/products/software/instrument-software/bd-facsdiva-software#Overview
FlowJo 2006-2015	FlowJo LLC	https://www.flowjo.com
Prism v7	GraphPad	https://www.graphpad.com/