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. 2022 Sep 12;13:5341. doi: 10.1038/s41467-022-33041-1

Fig. 1. ZIKV infection of human moDCs reprograms expression of lipid-related genes.

Fig. 1

a Human moDCs from four different donors were infected for 24 h with ZIKV SD001 at MOI 0.5. Cells were then stained for the viral envelope (E) protein using mAb 4G2 and sorted into ZIKV-infected (ZIKV+) and bystander uninfected (ZIKV−) cells. Total RNA was isolated and subjected to RNA-seq. Mock-infected cells (Mock) were analyzed in parallel. b Percent of reads aligned to the ZIKV genome in each cell population. c Venn diagram showing the number of unique and shared genes upregulated (fold-change >2, false discovery rate <0.01) in ZIKV+ vs ZIKV− or Mock moDCs. d Gene ontology analysis of genes significantly upregulated in ZIKV+ vs ZIKV− or Mock moDCs. e Heat map of the relative expression of selected genes implicated in lipid metabolism. f qRT-PCR analysis of the relative expression of fatty acid (FASN and SCD) and cholesterol (DHCR7 and HMGCR) synthesis genes. Data are presented as the mean ± SD. n = 4 (b) and n = 3 (f) biologically independent experiments. Symbols represent moDCs derived from individual donors. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s correction for multiple comparisons. Source data and exact P values are provided as a Source Data file.