Fig. 3. ZIKV infection of human moDCs induces de novo expression of lipid-related genes.

a moDCs from 3 donors were infected for 24 h with ZIKV SD001 (MOI 0.5) and cells were FACS sorted into ZIKV− and ZIKV+ populations. RNA from Mock, ZIKV−, and ZIKV+ moDCs were subjected to csRNA-seq. b Number of upregulated (fold-change >2, false discovery rate <0.01) TSRs. c Most enriched Metascape pathways associated with genes containing upregulated promoter proximal TSRs in ZIKV+ moDCs vs ZIKV− or Mock moDCs. d De novo motif analysis of TSRs upregulated in ZIKV+ moDCs vs ZIKV− moDCs. e Heat map of csRNA-seq reads at the TSRs of selected genes implicated in the lipid metabolism. f UCSC browser visualization of the SCD locus with RNA-seq and csRNA-seq in Mock, ZIKV−, and ZIKV+ moDCs. RNA-seq and csRNA-seq are strand-specific, with positive- and negative-strand transcription displayed above and below the central line, respectively.