FIGURE 4.

The LXC compounds inhibit GTPase activity in a dose-dependent manner. (A–F) The GTPase activity of purified AbFtsZ was determined from the liberation of P_i from GTP using malachite green. AbFtsZ was incubated with the LXC compounds for 30 min before the reaction was initiated by addition of 1 mM GTP. The reaction was terminated by the addition of citric acid after 10 min. P_i release rate was normalized to the sample without the addition of LXC compounds (86.26 μM P_i/mg AbFtsZ/min) that was taken as 100%. The results are presented in mean ± SEM. Statistical analysis was performed using one-way ANOVA (statistically significance, p < 0.05). A non-linear regression model was used in GraphPad Prism 8.3 to determine the IC₅₀.