FIGURE 4.

Abnormal gene expression of SCNT embryos at the 8‐cell stage. (A) Fuzzy c‐means (FCM) clustering analysis was used to divide the DEGs among adjacent developmental stages in IVF embryos and SCNT embryos into five clusters, respectively. (B) Boxplot showing the differential expression patterns of I_cluster 4 of IVF embryos between the two types of embryos. p < 0.001 taken as being statistically significant using Student's t‐test and denoted as ***. (C) The 22 most significant genes (power > 0.4, Avg_logFC > 1) in IVF embryos from I_cluster 4 selected by Seurat. (D) Only 6 out of the 22 most significance genes from I‐cluster 4 DEGs of IVF embryos were specifically expressed at the 8‐cell stage of SCNT embryos. (E) Three protein interaction networks based on I_cluster 4 were found by string (functional protein association networks) analysis