TABLE 4.
Fermentation results of H16 for PHB accumulation using high-copy-number plasmid a .
| Strains | WT | H16 | b percentage of increase (%) |
|---|---|---|---|
| Cell dry weight (g/L) | 5.01 ± 0.38 | 7.57 ± 0.18 | 51.1 ± 3.7 |
| Glucose utilization rate (g/L/h) | 0.54 ± 0.05 | 0.74 ± 0.01 | 36.1 ± 1.0 |
| Glucose consumption (g/L) | 29.0 ± 2.1 | 35.0 ± 0.71 | 20.7 ± 2.4 |
| PHB content (wt%) | 22.4 ± 3.5 | 34.3 ± 0.99 | 53.2 ± 4.4 |
| PHB concentration (g/L) | 1.13 ± 0.24 | 2.60 ± 0.10 | 130.1 ± 9.1 |
| Acetate concentration (g/L) | 18.41 ± 1.73 | 17.79 ± 0.69 | / c |
a
The original strain MG1655 (WT) and mutant H16 were cultivated in M9-basal medium supplemented with 30 g/L glucose at 37°C, 220 rpm, for 48 h. The Data are expressed as means (±s.d.) from three independent experiments.
b
The percentage of increase of H16 in cell dry weight, glucose utilization and PHB, accumulation compared with WT.
c
Not determined.