Table 3.

Relative abundance^* of phyla and genera after 72 h of ileal microbiota fermentation as the effect of BOT oil treatment, both unprotected (BOT) and microencapsulated (MBOT).

Treatment	Control	BOT_1.85	BOT_3.70	MBOT_3.50	MBOT_7.00	Colistin
Phylum
Firmicutes	0.92 ± 0.00a	0.82 ± 0.06a	0.02 ± 0.0.4c	0.94 ± 0.03a	0.91 ± 0.05a	0.22 ± 0.09b
Proteobacteria	0.04 ± 0.00bc	0.18 ± 0.06a	0.11 ± 0.0.4ab	0.06 ± 0.03bc	0.09 ± 0.05abc	0.00 ± 0.00c
Actinobacteria	0.04 ± 0.02b	0.00 ± 0.00b	0.87 ± 0.0a	0.00 ± 0.00b	0.00 ± 0.00b	0.78 ± 0.09a
Other	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
Genus
Bifidobacterium	0.04 ± 0.02b	0.00 ± 0.00b	0.87 ± 0.01a	0.00 ± 0.00b	0.00 ± 0.00b	0.78 ± 0.09a
Clostridium sensu stricto 1	0.01 ± 0.00b	0.00 ± 0.00b	0.00 ± 0.00b	0.00 ± 0.00b	0.00 ± 0.00b	0.20 ± 0.08a
Escherichia_Shigella	0.04 ± 0.01bc	0.17 ± 0.05a	0.11 ± 0.04ab	0.06 ± 0.03bc	0.09 ± 0.05abc	0.00 ± 0.00c
Lactobacillus	0.87 ± 0.03ab	0.81 ± 0.06b	0.02 ± 0.04c	0.94 ± 0.03a	0.91 ± 0.05ab	0.00 ± 0.00c
Streptococcus	0.03 ± 0.02a	0.00 ± 0.00b	0.00 ± 0.00b	0.00 ± 0.00b	0.00 ± 0.00b	0.02 ± 0.01ab
Others	0.00 ± 0.00	0.01 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00

^*Values are means ± Standard Deviation (SD) of triplicate determinations.

The different letters indicate significant differences (p < 0.05) between any pair of groups calculated by one-way ANOVA with Tukey's post-hoc test.