FIGURE 3.

Inhibition of shared neurons induced analgesia in CFA mice. (A) The representative pictures of DRG neurons expressing hM4D(Gi) receptors. The white arrows indicate double-labeled DRG neurons. As the EGFP signals were from original EGFP proteins expressed by the AAVRetro-hSyn-Cre-EGFP virus, while the mCherry proteins were expressed in a cre-dependent manner and their signals were amplified by the mCherry antibodies, all mCherry signal positive neurons were shared neurons. Bar = 150 μm. (B) The mechanical pain thresholds and thermal pain thresholds of the ipsilateral hind paw plantar were elevated after CNO treatment in the CFA model. n = 9, paired t-test. For mechanical pain thresholds, ^****P < 0.0001. For thermal pain thresholds, ^***P = 0.0004. (C) The mechanical pain thresholds and thermal pain thresholds of the ipsilateral hind paw plantar were increased a little after CNO treatment in normal mice. n = 6, paired t-test. For mechanical pain thresholds, *P = 0.0472. For thermal pain thresholds, *P = 0.0122.