TABLE 1.

Primers used

Primer	Sequencea	Remark
Inward facing
rdxA-F	5′-GCAGGAGCATCAGATAGTTCT-3′	886-bp product
rdxA-R	5′-GGGATTTTATTGTATGCTACAA-3′
rdxA-F1	5′-CGGACTCATGGAATTGCTCCAT-3′	1,343-bp product
rdxA-R1	5′-GGCAAATCATAGGCATTATGGTG-3′
Outward facing
rdxA-F2	5′-ATGGAAAAATTTCATTGATTTTCC-3′	601-bp deletion
rdxA-R2	5′-GCGATTACTTGGTTGTGATTAA-3′
rdxA-F3	5′-GTCATCTAGACCTGGCGATTTCAGCGATTTCT-3′	111-bp deletion
rdxA-R3	5′-GTCATCTAGAAGCGCTTCAGCGTTAATGGTGGT-3′
Inward facing
frxA-F1	5′-GCGCTTCAAAGCTTGGGTTACCA-3′	1,383-bp product
frxA-R1	5′-GCCTTCAATGTTGCGCTCTTTGT-3′
Outward facing
frxA-F2	5′-GGCGTGTCGGTAATGGCTTGTT-3′	523-bp deletion
frxA-R2	5′-GGTGCTGTAAAGCAACCACTTGT-3′
Inward facing
1508-F1	5′-CGGATCCAGCCACTCTAGCCA-3′	1,980-bp product
1508-R1	5′-GCACGCAAGACATTCGTTGCG-3′
Outward facing
1508-F2	5′-CCAAGGCTAGTGGTGATGA-3′	1,108-bp deletion
1508-R2	5′-GCACCAAGTGAGGATTGAAG-3′
Inward facing
588-F1	5′-GCGATTAGAGCCGTGATCTTC-3′	976-bp product
588-R1	5′-CGCATCACATCAGCGATCACT-3′
Outward facing
588-F2	5′-CATGCTCTTAGAAGAGACTA-3′	537-bp deletion
588-R2	5′-CCTTACACCTGTCTTCATTC-3′
Inward facing
cagA2143F	5′-GCAGAAGAAACGCTAAAAGCCCTT-3′	1,370-bp product
cagA3512R	5′-CTTCCCACATTATGCGCAACTAT-3′
Outward facing
cagA2900F	5′-CGATTGATGATCTCGGCGGACCTTT-3′	226-bp deletion
cagA2673R	5′-GGGTTCGTTTTCGAGTCCATTATTGT-3′

^aUnderline indicates XbaI site.