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. 2022 Aug 29;10:983031. doi: 10.3389/fcell.2022.983031

FIGURE 4.

FIGURE 4

In vivo characteristics of jejunum from 21-day-old suckling and 35-day-old weaned piglets. (A). Experimental design and method used for the culture of jejunum organoids from 21-day-old suckling and 35-day-old weaned piglets (n = 12 per group). (B). Histological observations, villus height and crypt depth of jejunum sections (stained with Alcian blue and Periodic Acid Schiff) from suckling and weaned piglets (n = 12 per group). Scale bars represent 100 µm. (C). Mean Log2 fold change of the relative abundance of metabolites in jejunum contents of weaned versus suckling piglets (n = 12 per group). Significant differences (p < 0.05) are indicated in blue (metabolites more abundant in weaned piglets) or in green (metabolites more abundant in suckling piglets). (D). Observed OTUs and Shannon a-diversity index and Non-Metric Dimensional Scaling (nMDS) two-dimensional representation of the microbiota ß-diversity using Bray-Curtis distance calculation (stress = 0.11) in the jejunum contents from suckling and weaned piglets (n = 12 per group). **: p < 0.01, ***: p < 0.001.