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Colocalized GFAP and GSNO expressions in the muscle layer. GFAP and GSNO fluorescence in different groups (A); green indicates GFAP, red indicates GSNO, and blue indicates DAPI-stained nucleus. One-way ANOVA indicated that the colocalization of GFAP and GSNO was not statistically different in different groups (B). Scale bar = 20 μm, bar: mean ± SE, n = 4.
