Simplex-lattice mixture design experiments for extraction of antioxidant compounds from defatted hemp seed and the correspondent responses.
| Run | Independent variables | Responses | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Water | Methanol | Acetone | TPCa | TACb | DPPHb | ABTSb | FRAPb | CUPRACb | |
| 1 | 1/3 | 2/3 | 0 | 22.56 | 166.38 | 13.32 | 75.83 | 31.02 | 33.38 |
| 2 | 2/3 | 1/3 | 0 | 22.20 | 178.52 | 8.48 | 77.83 | 23.91 | 19.28 |
| 3 | 1 | 0 | 0 | 27.51 | 125.70 | 2.70 | 64.13 | 15.54 | 17.92 |
| 4 | 2/3 | 1/6 | 1/6 | 33.35 | 191.39 | 20.74 | 93.99 | 39.43 | 38.62 |
| 5 | 1/6 | 1/6 | 2/3 | 36.46 | 194.46 | 18.41 | 81.29 | 48.25 | 63.65 |
| 6 | 0 | 1 | 0 | 14.10 | 151.51 | 7.81 | 31.25 | 29.80 | 41.51 |
| 7 | 0 | 0 | 1 | 6.86 | 41.24 | 0.92 | 19.27 | 12.21 | 22.13 |
| 8 | 1/3 | 1/3 | 1/3 | 38.80 | 217.66 | 25.18 | 117.47 | 45.38 | 65.97 |
| 9 | 0 | 1/3 | 2/3 | 13.52 | 119.61 | 5.71 | 30.33 | 20.67 | 37.21 |
| 10 | 1/3 | 0 | 2/3 | 45.81 | 247.89 | 34.15 | 122.37 | 61.01 | 84.92 |
| 11 | 2/3 | 0 | 1/3 | 50.19 | 231.42 | 26.91 | 117.41 | 51.65 | 56.33 |
| 12 | 1/6 | 2/3 | 1/6 | 27.79 | 163.40 | 15.31 | 79.44 | 38.57 | 43.88 |
| 13 | 0 | 2/3 | 1/3 | 20.01 | 172.27 | 9.52 | 49.81 | 17.83 | 43.29 |
| 14 | 1/3 | 1/3 | 1/3 | 41.89 | 237.60 | 25.61 | 105.41 | 48.34 | 56.27 |
a
Total phenolic content (TPC) is expressed in mg gallic acid equivalent per g of extract (mg GAE per g).
b
Total antioxidant capacity (TAC), cupric reducing antioxidant capacity (CUPRAC), Ferric reducing antioxidant power (FRAP), ABTS-, and DPPH- radical scavenging are expressed in mg torolox equivalent per g of extract (mg TE g−1).