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Efficiency of GLUT1 overexpression in isogenic MDA-MB-231 and MRC-5 derivative lines. (a) Western blot of GLUT1-transduced vs Turbo-635-transduced control cell lines. β-Actin is used as a loading control. (b) Glucose uptake capacity of the transduced cell lines as indicated by the relative uptake of fluorescent glucose analog 2-NBDG (100 μM, 20 min). Experiments were conducted in triplicate and the values shown are averages ± standard deviation. ** p < 0.01, *** p < 0.001.
