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. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: Wiley Interdiscip Rev RNA. 2022 Feb 14;13(5):e1717. doi: 10.1002/wrna.1717

Figure 6: Regulation of tRNA splicing.

Figure 6:

A) A cartoon of how CLP1 may modify splicing intermediates in which CLP1, a 5′-hydroxyl polynucleotide kinase, results in phosphorylation of the 5′-hydroxyl on the 3′-exon and the intron. The phosphorylated products are then unable to be ligated by the tRNA ligase RTCB, preventing the formation of mature tRNA and tricRNAs. At the bottom, it is shown that the phosphorylated intron may be targeted for kinase mediate intron degradation by a 5′-3′-exonuclease. B) A cartoon of how ANGEL2 may modify splicing intermediates. ANGEL2, as a 2′,3′-cyclic phosphatase, is believed to open the 2′,3′-cyclic phosphate on the 5′-exon and the intron, resulting in 2′ and 3′-hydroxyls and liberating a phosphate. These hydroxyl ends prevent the ligation of the splicing intermediates by the tRNA ligase RTCB, and thus preventing the formation of mature tRNA and tricRNAs. This figure was created with BioRender.com.