FIGURE 2.

OPTN expression affects senescence and ECM homeostasis of H₂O₂-teated rat NPCs. (A–B) The expression of OPTN after same overexpression lentivirus transfection with different MOI (OPTN-oe1:15, OPTN-oe2:30, OPTN-oe3:60). (C–E) Western blot and quantification of Aggrecan, MMP-1, Collagen Ⅱ, TIMP1, P21, P16 in overexpression lentivirus transfected rat NPCs followed by H₂O₂ treatment. (F–G) Sa-β-gal staining was used to detect the senescence of overexpression lentivirus transfected NPCs followed by H₂O₂ treatment (scale bar: 50 μm). (H–I) The expression of OPTN after knockdown lentivirus transfection. (J–L) Western blot and quantification of Aggrecan, MMP-1, Collagen Ⅱ, TIMP1, P21, P16 in knockdown lentivirus transfected rat NPCs followed by H₂O₂ treatment. (M–N) Sa-β-gal staining was used to detect the senescence of knockdown lentivirus transfected NPCs followed by H₂O₂ treatment. All experiments were performed as means ± SD of 3 times in duplicates (scale bar: 50 μm). *p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05.