FIGURE 4.

Mitophagy attenuates H₂O₂-induced rat NPCs damage by scavenging mtROS. (A–B) MitoSOX staining was used to detect mtROS in rat NPCs (scale bar: 50 μm). NPCs were treated with or without H₂O₂ conditions in the presence of CCCP, CsA. C–E) Western blot and quantification of Aggrecan, MMP-1, Collagen Ⅱ, TIMP1, P21, P16 in rat NPCs. NPCs were treated with or without H₂O₂ conditions in the presence of mitoTEMPO, CsA. (F–G) Sa-β-gal staining and quantification (scale bar: 50 μm). All experiments were performed as means ± SD of 3 times in duplicates. (n = 10). *p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05.