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Upregulation of OPTN alleviated IVDD in rats. (A–B) MRI and Pfirrmann grade of rat tail discs at 0 and 8 weeks after surgery (white arrows). (C–D) The X-ray and DHI% of rat tail discs at 8 weeks after surgery. (E–F) Representative HE, Safranin O-fast green staining of NP tissues and histological grades evaluated at 8 weeks in each group. All experiments were performed as means ± SD of 3 times in duplicates. (n = 10). *p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05.
