FIG. 4.

Delayed cold induction of cspL mRNA level with decreasing temperatures. RNA was extracted from an exponentially growing NC8 culture (OD₆₀₀ = 0.8) before and after different times following a temperature downshift from 28°C to 15, 10, 8, or 6°C. The resulting RNA samples were analyzed by hybridization with a cspL probe as described for Fig. 3. As the smaller transcript was barely detectable, quantification was performed only on the large transcript.