TABLE 2.
Summary of synaptosomes studies.
| Article | Population characteristics | [Ouabain] | [Ca2+]i levels, uptake | Major article findings in the domain of ouabain use |
|---|---|---|---|---|
| Satoh and Nakazato, 1989 | • Brain cortex of Sprague-Dawley rats of either sex | 5 × 10−8-5x10−4 M | = [Ca2+]i level (in absence of extracellular Ca2+) | Ouabain had no detectable effect on [Ca2+]i in the absence of extracellular Ca2+. However, it induced ACh release from synaptosomes, regardless of the presence or absence of extracellular Ca2+, which release impaired when the protein kinase C (PKC) and ryanodine receptor blocker was coincubated with ouabain |
| • 220–350 g | ||||
| Adam-Vizi and Ligeti (1986) | • Rat brain cortex of CFY rats | 5 × 10−5-5x10−4 M | = Ca Uptake and Efflux | Neither Ca2+ influx nor Ca2+ efflux was changed by ouabain. A slight increase of the uptake was only evoked by ouabain at a high concentration (>1 mM; data not shown) |
| • 120–150 g | ||||
| • n = 1-3 experiment made in duplicate | ||||
| Goddard and Robinson (1976) | • Rat male brains | 1 × 10−4 M | ↑122.0–185.0% Ca Uptake | Ouabain leads to an increase in uptake of45Ca, a high level of total calcium content and effectively prevents45Ca exit. The increased uptake of45Ca induced by ouabain was inhibited by voltage-gated sodium channels inhibitor and an inhibitor of intracellular calcium release by ryanodine receptors |
| • 200–300 g | ↑32.0% [Ca2+]i level | |||
| • n = 3-6 | ↑32.0% retention | |||
| Swanson et al. (1974) | • Rat brain | 1 × 10−4 M | ↑33.0% Ca Uptake | OUA stimulated Ca uptake by synaptosomes |
| • n = 3-4 | ||||
| Blaustein and Wiesmann (1970) | • Rat brain | 1 × 10−3 M | ↑113.0% Ca Uptake | Calcium influx is increased when the internal sodium concentration is increased by treatment with ouabain |
| • 200–250 g | ||||
| • n = 3 |