FIG. 4.

Effects of disruption of DOG2 and DOG1 on susceptibility to various stresses. (A) Cells were grown in fructose medium (2% fructose, 0.67% yeast nitrogen base without amino acids supplemented with appropriate amino acids and bases; pH 5.5) at 28°C with shaking for 16 h. A small portion of the culture was transferred to fresh fructose medium containing 0.4 mM t-BHP or 0.1% 2-deoxyglucose (2-deGlc) and cultured at 28°C to monitor the OD₆₁₀. Strains used are as follows: open triangles, YPH252 (wild type); open circles, SET8-1-C (dog2); closed circles, SCDG1 (dog1 dog2). (B) Cells (10⁵ cells/ml) were spotted (5 μl) on YPD agar plates with or without 0.9 M KCl and incubated at 28°C for 2 days. Strains used are as follows: wild type (WT), YPH252; dog2, SET8-1-C; dog1 dog2, SCDG1; pbs2, YPB2; hog1, YHG1.