Skip to main content
. Author manuscript; available in PMC: 2022 Sep 12.
Published in final edited form as: Nature. 2020 Jun 3;582(7812):395–398. doi: 10.1038/s41586-020-2348-z

Figure 1. Neuroprotection in rodent models of stroke.

Figure 1.

a. Opposite circadian cycles in nocturnal rodents versus diurnal humans. b. 100 min middle cerebral artery occlusion (MCAO) in male Sprague-Dawley rats. Controls received 30% O2. NBO group received 100% O2 (for 85% of ischemic period). NBO reduced infarction at ZT3-9 but not ZT15-21. c. NBO (for 85% of ischemic period) reduced infarction after 60 min MCAO in Sprague-Dawley rats during ZT3-9. d. αPBN (100 mg/kg IP immediately and 2 hrs post-reperfusion) reduced infarction after 60 min MCAO in ZT3-9 but not ZT15-21 mice. e. MK801 (2.5 mg/kg IP, 1 hr pre-occlusion) reduced infarction after permanent MCAO in ZT3-9 but not ZT15-21 mice. f. Infarct volumes were smaller in 60 min transient versus permanent MCAO in both ZT3-9 and ZT15-21 mice. All values in Figure 1 are mean ± SEM; comparisons via 2-tailed t-test. Physiologic parameters, laser-doppler flow, inclusion/exclusion/mortality in Extended Data 1. White bars: ZT3-9; gray bars: ZT15-21. Infarct volumes quantified with triphenyltetrazolium (TTC) staining. (n) indicate animals per group. tMCAO: transient MCAO; pMCAO: permanent MCAO; dMCAO permanent distal MCAO.