TABLE 1.
Bacterial strains, plasmids, and bacteriophage used in this study
Strain, plasmid, or phage | Description | Reference or source |
---|---|---|
Bacterial strains | ||
E. coli | ||
DH5α | F− φ80d lacZΔM15 ΔlacZYA-argFU169 recA1 endA1 supE44 | 22 |
DH10B | F−mcrA Δmrr-hsdRMS-mcrBCφ80d lacZΔM15 ΔlacX74 endA1 recA1 deoRΔara leu 7697 araD139 galU galK nupG rpsL λ− | 31 |
DF1 | γδ transposase tnpA | Gibco BRL |
MC1061 | sup0hsdR mcrB araD139 ΔaraABC-leu7679 ΔlacX74 galU galK rpsL thi | 7 |
MC4100 | araD139 ΔlacZYA-argFU169 rpsL150 StrrelA1 flbB5301 deoC1 ptsF25 rbsR | 38 |
XL1-BlueMRA | ΔmcrA183 ΔmcrCB-hsdSMR-mrr-173 endA1 supE44 thi-1 reA1 gyrA96 relA1 | Stratagene |
S. entomophila | ||
A1MO2 | Apr, pADAP, pathogenic | 19 |
5.6 | Heat-cured pADAP-minus derivative of A1MO2 | 17 |
5.6RC | CmrrecA pADAP-minus strain | 20 |
5.6RK | KnrrecA pADAP-minus strain | This study |
Plasmids | ||
pACYC184 | Cmr Tcr | 8 |
pADAP | Amber disease-associated plasmid | 17 |
pBR322 | Apr Tcr | 3 |
pBM32 | 23-kb BamHI fragment from pMH32 cloned in pBR322 | This study |
pBM32-1-40 | pBM32 containing mini-Tn10 insertions | This study |
pDELTA1 | Apr Smr Knr, sucroser | Gibco BRL |
pLAFR3 | Tcr pRK290 with λcos, lacZα, and multicloning site from pUC8 | 39 |
pGLA20 | 11-kb HindIII pADAP fragment cloned in pLAFR3 | 20 |
pADK-13 | pADAP::mini-Tn10 insertion in 10.6-kb HindIII fragment, Knr, nonpathogenic | 20 |
pADK-35 | pADAP::mini-Tn10 insertion in 10.6-kb HindIII fragment, Knr, pathogenic | 20 |
pMH32 | 23-kb BamHI fragment of pADAP cloned into pLAFR3 | This study |
pMH41 | 33-kb BamHI fragment of pADAP cloned into pLAFR3 | This study |
pBM32 | 23-kb BamHI fragment of pMH32 cloned into pBR322 | This study |
pUC19 | Apr, lacZα, multicloning site | 42 |
Bacteriophage | ||
λNK1316 | Mini-Tn10 derivative 103 donor λb522 cI857 Pam80 nin5 | 27 |