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. 2000 Sep;182(18):5127–5138. doi: 10.1128/jb.182.18.5127-5138.2000

TABLE 2.

Disease-causing ability of the cloned virulence-encoding region, its mutated derivatives, and pADAP recombined mutationsa

Positive (A1MO2), 91–100%b
Negative (untreated carrot), 5.3–22.8%b
Construct Mean % showing disease symptoms One-tailed t testc
Construct Mean % showing disease symptoms One-tailed t test
df P df P
Virulence-encoding clones
 pBM32 97.8 3 NV pMH32 97.0 2 0.211
 pMH41 95.5 1 0.250
pBM32 derivativesd
 pBM32-1 97.0 2 0.500 pBM32-21 88.7 2 0.211
 pBM32-2 16.5 1 0.052 pBM32-22 8.3 2 0.005*
 pBM32-3 4.0 1 0.015* pBM32-23 91.5 3 0.196
 pBM32-4 12.50 1 0.050 pBM32-24 94.0 2 0.500
 pBM32-5 25.0 pBM32-25 94.0 2 0.211
 pBM32-6 4.0 1 0.029* pBM32-26 93.3 3 NV
 pBM32-7 8.0 pBM32-27 10.5 1 0.041*
 pBM32-8 94.0 2 0.211 pBM32-28 94 2 0.001*
 pBM32-9 93.3 3 0.091 pBM32-29 8.0 1 NV
 pBM32-10 95.8 5 0.298 pBM32-30 8.0 1 NV
 pBM32-11 2.9 3 0.029* pBM32-31 10.5 1 0.056
 pBM32-12 18.5 1 0.011* pBM32-32 10.7 2 0.002*
 pBM32-13 94.0 2 0.500 pBM32-33 91.0 4 0.035*
 pBM32-14 8.0 1 0.031* pBM32-34 0.0 2 0.000*
 pBM32-15 95.5 1 NV pBM32-35 87.5 3 0.187
 pBM32-16 100.0 pBM32-36 97.0 2 0.211
 pBM32-17 21.0 pBM32-37 2.7 2 0.000*
 pBM32-18 91.0 1 0.250 pBM32-38 4.0 1 0.015*
 pBM32-19 8.0 pBM32-39 4.0 1 0.029*
 pBM32-20 93.0 4 0.102 pBM32-40 0.0 1 NV
pADAP pADK derivativese
 pADK-1 97.0 2 0.211 pADK-21 94.0 2 0.092
 pADK-4 8.3 (NF) 2 0.004* pADK-22 12.0 (NF) 1 0.002*
 pADK-5 12.0 (NF) 1 0.014* pADK-23 93.3 3 0.091
 pADK-6 4.0 (NF) 1 0.013* pADK-24 91.0
 pADK-8 8.0 (NF) pADK-30 13.7 (NF) 2 0.006*
 pADK-9 97.0 2 0.211 pADK-31 4.0 (NF) 1 0.013*
 pADK-10 88.7 2 0.131 pADK-32 19.0 (NF) 2 0.004*
 pADK-11 12.0 (NF) 1 0.014* pADK-34 13.3 (NF) 2 0.000*
 pADK-12 95.5 1 0.250 pADK-35 100.0 3 NV
 pADK-13 97.8 3 0.196 pADK-39 8.0 (NF) 1 0.028*
a

NF, larvae were healthy in appearance but unable to feed. 

b

Range of controls over 80 batches. 

c

One-tailed paired t test over batches of the percentage of C. zealandica larvae showing full disease symptoms (amber coloration and inhibition of feeding) after ingestion of Serratia entomophila at day 12. NV, no variation; ∗, significant difference (P < 0.05); —, only one batch. 

d

See Fig. 1C for locations of pBM32 mutations. 

e

See Fig. 1D for locations of pADK mutations. 

HHS Vulnerability Disclosure