TABLE 2.
Disease-causing ability of the cloned virulence-encoding region, its mutated derivatives, and pADAP recombined mutationsa
Positive (A1MO2), 91–100%b
|
Negative (untreated carrot), 5.3–22.8%b
|
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---|---|---|---|---|---|---|---|
Construct | Mean % showing disease symptoms | One-tailed t testc
|
Construct | Mean % showing disease symptoms | One-tailed t test
|
||
df | P | df | P | ||||
Virulence-encoding clones | |||||||
pBM32 | 97.8 | 3 | NV | pMH32 | 97.0 | 2 | 0.211 |
pMH41 | 95.5 | 1 | 0.250 | ||||
pBM32 derivativesd | |||||||
pBM32-1 | 97.0 | 2 | 0.500 | pBM32-21 | 88.7 | 2 | 0.211 |
pBM32-2 | 16.5 | 1 | 0.052 | pBM32-22 | 8.3 | 2 | 0.005* |
pBM32-3 | 4.0 | 1 | 0.015* | pBM32-23 | 91.5 | 3 | 0.196 |
pBM32-4 | 12.50 | 1 | 0.050 | pBM32-24 | 94.0 | 2 | 0.500 |
pBM32-5 | 25.0 | — | pBM32-25 | 94.0 | 2 | 0.211 | |
pBM32-6 | 4.0 | 1 | 0.029* | pBM32-26 | 93.3 | 3 | NV |
pBM32-7 | 8.0 | — | pBM32-27 | 10.5 | 1 | 0.041* | |
pBM32-8 | 94.0 | 2 | 0.211 | pBM32-28 | 94 | 2 | 0.001* |
pBM32-9 | 93.3 | 3 | 0.091 | pBM32-29 | 8.0 | 1 | NV |
pBM32-10 | 95.8 | 5 | 0.298 | pBM32-30 | 8.0 | 1 | NV |
pBM32-11 | 2.9 | 3 | 0.029* | pBM32-31 | 10.5 | 1 | 0.056 |
pBM32-12 | 18.5 | 1 | 0.011* | pBM32-32 | 10.7 | 2 | 0.002* |
pBM32-13 | 94.0 | 2 | 0.500 | pBM32-33 | 91.0 | 4 | 0.035* |
pBM32-14 | 8.0 | 1 | 0.031* | pBM32-34 | 0.0 | 2 | 0.000* |
pBM32-15 | 95.5 | 1 | NV | pBM32-35 | 87.5 | 3 | 0.187 |
pBM32-16 | 100.0 | — | pBM32-36 | 97.0 | 2 | 0.211 | |
pBM32-17 | 21.0 | — | pBM32-37 | 2.7 | 2 | 0.000* | |
pBM32-18 | 91.0 | 1 | 0.250 | pBM32-38 | 4.0 | 1 | 0.015* |
pBM32-19 | 8.0 | — | pBM32-39 | 4.0 | 1 | 0.029* | |
pBM32-20 | 93.0 | 4 | 0.102 | pBM32-40 | 0.0 | 1 | NV |
pADAP pADK derivativese | |||||||
pADK-1 | 97.0 | 2 | 0.211 | pADK-21 | 94.0 | 2 | 0.092 |
pADK-4 | 8.3 (NF) | 2 | 0.004* | pADK-22 | 12.0 (NF) | 1 | 0.002* |
pADK-5 | 12.0 (NF) | 1 | 0.014* | pADK-23 | 93.3 | 3 | 0.091 |
pADK-6 | 4.0 (NF) | 1 | 0.013* | pADK-24 | 91.0 | — | |
pADK-8 | 8.0 (NF) | — | pADK-30 | 13.7 (NF) | 2 | 0.006* | |
pADK-9 | 97.0 | 2 | 0.211 | pADK-31 | 4.0 (NF) | 1 | 0.013* |
pADK-10 | 88.7 | 2 | 0.131 | pADK-32 | 19.0 (NF) | 2 | 0.004* |
pADK-11 | 12.0 (NF) | 1 | 0.014* | pADK-34 | 13.3 (NF) | 2 | 0.000* |
pADK-12 | 95.5 | 1 | 0.250 | pADK-35 | 100.0 | 3 | NV |
pADK-13 | 97.8 | 3 | 0.196 | pADK-39 | 8.0 (NF) | 1 | 0.028* |
NF, larvae were healthy in appearance but unable to feed.
Range of controls over 80 batches.
One-tailed paired t test over batches of the percentage of C. zealandica larvae showing full disease symptoms (amber coloration and inhibition of feeding) after ingestion of Serratia entomophila at day 12. NV, no variation; ∗, significant difference (P < 0.05); —, only one batch.
See Fig. 1C for locations of pBM32 mutations.
See Fig. 1D for locations of pADK mutations.