TABLE 1.
Purification of glucan glucohydrolase from T. neapolitana
| Purification step | Total protein (mg) | Total activitya (U) | Sp act (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Crude extract | 2,425 | 4,994 | 2.06 | —b | —b |
| Acidification to pH 4.3 (supernatant) | 628 | 2,747 | 4.37 | 1 | 100 |
| Anion-exchange chromatography (Q-Sepharose) | 152 | 2,200 | 14.47 | 3 | 80 |
| Gel filtration (Biogel P-60) | 82.8 | 2,025 | 24.45 | 6 | 74 |
| Hydrophobic-interaction chromatography (phenyl-Sepharose) | 24.4 | 1,735 | 71.1 | 16 | 63 |
| Affinity chromatography (galactose-agarose) | 3 | 1,460.9 | 374.3 | 86 | 53 |
| Anion-exchange chromatography (Mono-Q) | 3.2 | 1,413 | 441.5 | 97 | 51 |
| Preparative PAGE (native) | 1.1 | 888 | 807.27 | 184 | 32 |
a
The substrate used in enzyme assays was oNPG.
b
Purification and recovery were not calculated because the crude extract contains at least two enzymes with aryl-glycosidase activity.