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. 2022 Feb 1;18(9):2161–2177. doi: 10.1080/15548627.2021.2022289

Figure 2.

Figure 2.

Perinatal deletion of ULK2 leads to a compensatory increase in basal autophagy. All data was obtained in 10- to 14-wk-old mice unless otherwise specified. (A) Constitutive ULK2 deletion in isolated cardiomyocytes of cU2-KO mice. (B) Protein levels of MAP1LC3A-MAP1LC3B (MAP1LC3), SQSTM1, and NBR1 in randomly fed U2 fl/fl and cU2-KO mice. Representative immunoblots (Left) and quantification of relative expression (Right) (n = 4–7). (C) Protein levels of MAP1LC3 analyzed after chloroquine administration. Representative immunoblots (Top) and summary of relative expression (Bottom) (n = 6–10). (D) Representative B-mode images and serial cardiac function of U2 fl/fl and cULK2-KO mice measured at 10, 38, 64, and 86 weeks of age (n = 5–13). (E) Heart weight:tibia length reveals mild cardiac hypertrophy in cU2-KO mice (n = 5–6). (F) Representative H&E and Masson’s Trichrome images (Left) (scale: 20 μm) and quantification of fibrotic tissue (Right) (n = 4–6). Data are means ± SEM; *P < 0.05.