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. 2022 Feb 1;18(9):2161–2177. doi: 10.1080/15548627.2021.2022289

Figure 6.

Figure 6.

Adult deletion of ULK1 rapidly impairs cardiac autophagy. Data were obtained in 9-wk old mice 4 days after initial tamoxifen injection, unless otherwise specified. (A) Protein levels of MAP1LC3A-MAP1LC3B (MAP1LC3) analyzed after chloroquine administration. Representative immunoblots (Left) and quantification of relative expression (Right) (n = 5–6). (B) Representative TEM images (8000x magnification) of U1fl/fl and cU1-KO hearts treated with vehicle (VEH) or chloroquine (CQ) (Top). Red arrowheads indicate autophagosomes and red arrows indicate autolysosomes. Quantification of autophagosomes and autolysosomes relative to U1 fl/fl treated with vehicle (Bottom) (n = 4). (C) Representative TEM images (3x zoom of original 2000x magnification) of U1 fl/fl and icU1-KO hearts treated with vehicle (VEH) or chloroquine (CQ) (scale bar: 1 μm). Red arrows indicate mitophagosomes. Quantification of mitophagosomes relative to U1 fl/fl treated with vehicle (Bottom) (n = 4). Data are means ± SEM; *P < 0.05, **P < 0.01 and ***P < 0.001.