FIG. 2.

Growth requirements of gshA mutations in S. enterica. Soft agar containing strain DM4620 (gshA) was overlaid on minimal glucose medium. Compounds were spotted in 2-μl aliquots on the solidified agar as indicated, at the following concentrations: thiamine (B₁), HMP, and THZ, 100 μM; GSH and γ-GC, 10 mM.