TABLE 1.

Summary of the immunological reactivities of cleavage bands produced from NtrC^D86CFe and NtrC^{D86CFe,Δ444–469a}

Band	Band detected with:
	Anti-MBP	Anti-N-ter	Anti-C-ter	Anti-metal chelate
1b	+	+	−	+
2c	+	+	−	+
2′c	−	+	+	+
3	+	+	−	−
4	−	+	+	−
5b	−	−	+	−

^aAnalysis was performed by Western blotting using antisera directed against MBP, the N-terminal domain of NtrC (N-ter), the C-terminal domain of NtrC (C-ter), intact NtrC, or the metal chelate (CHA255) (see Materials and Methods). Antibodies directed against full-length NtrC detected all bands detected by other antibodies and no additional bands. The latter confirmed that there were no cleavage fragments that lacked both termini of NtrC. 

^bProduced only when proteins were phosphorylated. 

^cSeparated only in NtrC^{D86CFe,Δ444–469.}