TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| B. subtilis strains | ||
| CU1065 | W168 trpC2 attSPβ | Lab stock |
| HB7070 | CU1065 SPβ7063 [PW-cat-lacZ] | 22 |
| HB0011 | HB7070 rsiW::Km | This work |
| HB7022 | CU1065 SPβ7019 [PX-cat-lacZ] | 21 |
| HB7024 | HB7022 rsiX::pVA29 | 21 |
| OKB105 | pheA1 sfp | 39 |
| HB300 | CU1065 yqgE::pMUTIN (MLSr) | This study |
| HB301 | HB7070 pXT-yshB′ (Spcr MLSr Neor) | This study |
| HB302 | CU1065 yjdD::pMUTIN (MLSr) | This study |
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 53 |
| Jm2r− | mcrAB hsdR recA1 Δ(lac-proAB) thi gyrA96 relA1 srl::Tn10 F′ (proAB lacZ ΔM15) | S. Zahler |
| Plasmids | ||
| pIC333 | pUC replicon, shuttle vector carrying mini-Tn10, thermosensitive replicon for gram-positive hosts (Apr MLSr Spcr) | 57 |
| pMUTIN4 | pBR322-based integration vector for B. subtilis; contains a multiple cloning site downstream of the Pspac promoter (Apr MLSr) | 64 |
| pMUTIN-yqgE | pMUTIN4 derivative carrying DNA from the 5′ end of yqgE | This study |
| pMUTIN-yjdD | pMUTIN4 derivative carrying DNA from within yjdD | This study |
| pXT | Vector which integrates into the thrC locus and contains the xylA xylose-inducible promoter upstream of a multiple cloning site (Apr MLSr Spcr) | T. Msadek |
| pXT-yshB′ | pXT derivative carrying DNA from the 5′ end of yshB | This study |