TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| DH5-α | F′ lacZM15 recA1 | Life Technologies |
| S17-1 | recA integrated plasmid RP4 | 5 |
| YK4146 | fliC mutant | R. M. Macnab |
| YK4516 | fliC mutant | R. M. Macnab |
| R. sphaeroides | ||
| WS8N | Wild-type spontaneous Nalr | 37 |
| nm15b | TnphoA derivative of WS8N, nonmotile, nonflagellate | This study |
| Cosmids or plasmids | ||
| c523 | Cosmid carrying the R. sphaeroides fliC gene; Tcr | This study |
| pUC19 | E. coli-specific cloning vector lacZ Ampr | New England Biolabs |
| pSB390 | luxCDEAB cassette clone | G. S. A. B. Stewart and M. Winson |
| pSB395 | luxCDEAB cassette cloned in pRK415 | 41 |
| pTP11 | luxCDEAB cassette cloned in pRK415 under the control of the promoter region of fliC | This study |
| pRK415-1 | Cloning vector which can replicate in R. sphaeroides; Tcr | 36 |