FIG. 1.

Tnl0kan insertion site and expression of rpoE and htrA in WK3 (W3110 rseA::Tn10kan). (a) The insertion site of Tn10kan in WK3, the ribosome-binding sequence (RBS) of rseA, and the stop codon of rpoE are represented by a triangle, an underline, and an asterisk, respectively. Amino acid sequences of RpoE and RseA are shown under the nucleotide sequence. Two promoters, P1 and P2 (arrows attached to solid circles), exist for the rpoE-rseA-rseB-rseC (boxes) operon. (b) Expression of htrA (left) and rpoE (right) was analyzed by RT-PCR with total RNA from WK3 or the wild-type (WT) W3110 cells, which were grown until the stationary phase (24 h) under the conditions described in Materials and Methods. Cycles show the number of PCRs. In the left panel, the left and right arrowheads indicate the positions of RT-PCR products for the htrA and rpoE transcripts, respectively. The right panel represents rRNAs used as a control.