| Recombinant DNA reagent |
pACEbac1 Smc1 Smc3 Scc1-StrepII |
This Study |
pJC 93 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 G22C K639C Smc3 E570C S1043C Scc1-StrepII A547C |
This Study |
pJC 95 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 K639C N1192C Smc3 E570C R1222C Scc1-StrepII |
This Study |
pJC 153 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 G22C N1192C Smc3 S1043C R1222C Scc1-StrepII A547C |
This Study |
pJC 154 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 G22C K639C Smc3Scc1-StrepII E570C A547C |
This Study |
pJC 127 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 G22C N1192C Smc3 S1043C R1222C Scc1-StrepII A547C |
This Study |
pJC 123 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 G22C L597ST Smc3 S1043C S606SC Scc1-StrepII A547C |
This Study |
pJC 106 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc3Scc1Smc1-StrepII E570C K639C |
This Study |
pJC 132 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc1 G22C L597ST Smc3Scc1-StrepII S606SC A547C |
This Study |
pJC 129 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc3 S1043C S606SC Scc1Smc1-StrepII L597ST |
This Study |
pJC 124 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Smc3Scc1Smc1-StrepII S606SC TEV R1222C L597ST N1192C |
This Study |
pJC 135 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1i Smc1-His E1102C Smc3 Scc1-StrepII |
This Study |
pJC 151 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1i Smc1 Smc3 S72C Scc1-StrepII |
This Study |
pJC 100 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1i Smc3 K1004C Smc1 _Scc1-StrepII |
This Study |
pJC 101 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1i Smc1 Smc3 S72C K1004C Scc1-StrepII |
This Study |
pJC 102 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1i Smc1 E1102C N1192C Smc3 K1004C R1222C Scc1-StrepII |
This Study |
pJC 105 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Scc2133-1493-StrepII |
Collier et al., 2020
|
pJC 69 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Scc2133-1493-StrepII T1281C |
This Study |
pJC 75 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Scc2133-1493-StrepII E819C |
This Study |
pJC 77 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Scc2133-1493-StrepII D369C |
This Study |
pJC 76 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Scc2133-1493-StrepII D369C E819C |
This Study |
pJC 78 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 Scc2133-1493-StrepII D369C T1281C |
This Study |
pJC 80 |
Plasmid obtainable from Nasmyth Lab |
| Recombinant DNA reagent |
pACEbac1 StrepII-Scc3 |
Collier et al., 2020
|
pJC 84 |
Plasmid obtainable from Nasmyth Lab |
| Chemical compound, drug |
ATP |
Sigma |
Cat # 11140965001 |
|
| Chemical compound, drug |
BMOE |
ThermoFisher |
Cat # 22323 |
|
| Chemical compound, drug |
HRV 3 C protease |
Pierce |
Cat # 88946 |
|
| Chemical compound, drug |
TEV protease |
Invitrogen |
Cat # 12575023 |
|
| Chemical compound, drug |
EnzChek phosphate assay kit |
Invitrogen |
Cat # E6646 |
|
