Figure 1.

CD47 is dispensable for T cell development.

(a) Single cell suspensions from thymus of WT (Cd47^+/+) and KO (Cd47^−/-) littermate and sex-matched mice were FcR-blocked and stained for CD45.2, CD3, CD4, CD8, CD25, c-kit, and CD28. DAPI was used to discriminate live/dead cells. Live, CD45.2 cells were gated for CD4 and CD8 expression. CD4-CD8- double negative (DN) cells are further gated for c-Kit, CD25 and CD28 expression to distinguish among early thymic progenitor (ETP), double negative (DN)2, DN3, DN3a, DN3b1, DN3b2, and DN4 population, n = 5. (b) Thymus of 8 weeks old WT and Cd47^−/− littermate female mice are comparable in sizes, n = 5. (c) Frequencies of CD4+ and CD8+ single positive cells in thymus are comparable between WT and Cd47^−/- mice. n = 5. (d) Pan T cells were isolated from the thymus of WT and Cd47^−/- mice and incubated with anti-CD3 plus anti-CD28 on ice and then on 37°C for the indicated time. Cells were immediately fixed, permeabilized, and intracellular stained for anti-phosphotyrosine (pY). CD8+ T cell subsets were flow gated and analyzed for intracellular pY. Representative of two independent experiments.