Table 2.
List and characteristics of primers (F and R) and probes (P) used for Rt-qPCR analyses. From Corman et al. (2020). E: envelope protein gene; RdRp: RNA-dependent RNA polymerase gene; N or N1 (in wastewater): nucleocapsid protein gene.
| Medium | Gene | Oligonucleotide | Sequence | Final concentration |
|---|---|---|---|---|
| Mussels | RdRp | RdRp_SARSr-F | GTG-ARA-TGG-TCA-TGT-GTG-GCG-G | 600 nM |
| RdRp_SARSr-R | CAR-ATG-TTA-AAS-ACA-CTA-TTA-GCA-TA | 800 nM | ||
| RdRp_SARSr-P2 | CAG-GTG-GAA-CCT-CAT-CAG-GAG-ATG-C | 100 nM | ||
| E | E_Sarbeco_F | ACA-GGT-ACG-TTA-ATA-GTT-AAT-AGC-GT | 400 nM | |
| E_Sarbeco_R | ATA-TTG-CAG-CAG-TAC-GCA-CAC-A | 400 nM | ||
| E_Sarbeco_P1 | ACA-CTA-GCC-ATC-CTT-ACT-GCG-CTT-CG | 200 nM | ||
| N | N_Sarbeco_F | CAC-ATT-GGC-ACC-CGC-AAT-C | 600 nM | |
| N_Sarbeco_R | GAG-GAA-CGA-GAA-GAG-GCT-TG | 800 nM | ||
| N_Sarbeco_P | ACT-TCC-TCA-AGG-AAC-AAC-ATT-GCC-A | 200 nM | ||
| Water | RdRp | nCoV_IP4-14059Fw | GGT-AAC-TGG-TAT-GAT-TTC-G | 400 nM |
| nCoV_IP4-14146Rv | CTG-GTC-AAG-GTT-AAT-ATA-GG | 400 nM | ||
| nCoV_IP4–14084P | TCA-TAC-AAA-CCA-CGC-CAG-G | 200 nM | ||
| N1 | 2019-nCoV_N1–F | GAC-CCC-AAA-ATC-AGC-GAA-AT | 400 nM | |
| 2019-nCoV_N1-R | TCT-GGT-TAC-TGC-CAG-TTG-AAT-CTG | 400 nM | ||
| 2019-nCoV_N1–P | ACC-CCG-CAT-TAC-GTT-TGG-TGG-ACC | 200 nM |