Table 3.
Concentrations (gene copies/L) of the SARS-CoV-2 genome in raw wastewater from WWTPs in Reims and center Seine, averaged over the week or over the duration of exposure. The concentrations under the various dilution conditions are estimates. Data are expressed as mean ± standard deviation (SD). The concentration estimate for the dilution conditions were obtained with respect to the 100% condition. E: envelope protein gene; RdRp: RNA-dependent RNA polymerase gene; N1: nucleocapsid protein gene; NA: not analysed; DL: detection limit.
| Experiment | Condition/Week | RNA concentration in raw wastewater (average over the week or over the duration of the experiment) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
RdRp gene (copies/L) |
E gene (copies/L) |
N1 gene (copies/L) |
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| Mean | ± | SD | Mean | ± | SD | Mean | ± | SD | ||
| Spring caging (April–May 2020) | Reims WWTP (W15) | NA | ± | NA | 489,525 | ± | 411,855 | NA | ± | NA |
| Reims WWTP (W16) | NA | ± | NA | 464,844 | ± | 426,420 | NA | ± | NA | |
| Reims WWTP (W17) | NA | ± | NA | 21,891 | ± | 17,883 | NA | ± | NA | |
| Reims WWTP (W18) | 398 | ± | 406 | < DL | ± | < DL | NA | ± | NA | |
| Reims WWTP (W19) | 390 | ± | 202 | 20,634 | ± | NA | ± | NA | ||
| center Seine WWTP (W16) | NA | ± | NA | 223,704 | ± | 16,364 | NA | ± | NA | |
| center Seine WWTP (W17) | NA | ± | NA | NA | ± | NA | NA | ± | NA | |
| center Seine WWTP (W18) | NA | ± | NA | 44,178 | ± | NA | ± | NA | ||
| center Seine WWTP (W19) | NA | ± | NA | 2045 | ± | NA | ± | NA | ||
| Autumn caging (September-October-November 2020) | Reims WWTP (W39) | 39,276 | ± | 23,004 | 80,507 | ± | 39,936 | 141,385 | ± | 189,176 |
| Reims WWTP (W40) | 14,983 | ± | 12,229 | 71,795 | ± | 50,457 | 31,469 | ± | 19,527 | |
| Reims WWTP (W41) | 12,110 | ± | 6586 | 77,802 | ± | 55,418 | 32,343 | ± | 15,197 | |
| Reims WWTP (W42) | 5110 | ± | 2640 | 37,909 | ± | 22,827 | 19,212 | ± | 15,800 | |
| Reims WWTP (W43) | 8169 | ± | 5701 | 41,674 | ± | 19,787 | 21,552 | ± | 7286 | |
| Reims WWTP (W44) | 11,287 | ± | 2161 | 59,840 | ± | 19,573 | 36,114 | ± | 21,821 | |
| Reims WWTP (W45) | 12,417 | ± | 8961 | 73,853 | ± | 29,035 | 42,215 | ± | 16,489 | |
| Reims WWTP (W46) | 8388 | ± | 2969 | 40,722 | ± | 18,186 | 46,769 | ± | 39,254 | |
| Reims WWTP (W47) | 9156 | ± | 3901 | 79,050 | ± | 78,280 | 27,520 | ± | 19,756 | |
| Reims WWTP (W48) | 3814 | ± | 4826 | 91,106 | ± | 124,838 | 15,137 | ± | 11,540 | |
| 1st laboratory exposure (August 2020) | 100% raw wastewater | 6173 | ± | 2942 | 21,400 | ± | 12,297 | 24,157 | ± | 25,522 |
| 33% raw wastewater | 2037 | ± | 971 | 7062 | ± | 4058 | 7972 | ± | 8422 | |
| 10% raw wastewater | 617 | ± | 294 | 2140 | ± | 1230 | 2416 | ± | 2552 | |
| 2nd laboratory exposure (September 2020) | 100% raw wastewater | 4244 | ± | 421 | 17,237 | ± | 8801 | 30,611 | ± | 18,872 |
| 33% raw wastewater | 1401 | ± | 139 | 5688 | ± | 2904 | 10,102 | ± | 6228 | |
| 10% raw wastewater | 424 | ± | 42 | 1724 | ± | 880 | 3061 | ± | 1887 | |
| 3rd laboratory exposure (September 2020) | 100% raw wastewater | 32,230 | ± | 19,355 | 73,026 | ± | 41,874 | 57,763 | ± | 33,155 |
| 33% raw wastewater | 10,636 | ± | 6387 | 24,098 | ± | 13,818 | 19,062 | ± | 10,941 | |
| 10% raw wastewater | 3223 | ± | 1935 | 7303 | ± | 4187 | 5776 | ± | 3316 | |
| 4th laboratory exposure (November 2020) | 100% raw wastewater | 9913 | ± | 4086 | 113,242 | ± | 120,853 | 26,232 | ± | 18,650 |
| 33% raw wastewater | 3271 | ± | 1348 | 37,370 | ± | 39,882 | 8657 | ± | 6154 | |
| 10% raw wastewater | 991 | ± | 409 | 11,324 | ± | 12,085 | 2623 | ± | 1865 | |