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. 2022 Jul 1;21(19):2013–2026. doi: 10.1080/15384101.2022.2079175

Figure 1.

Figure 1.

LncRNA DLEU2, high-expressed in atherosclerosis, affected PDGF-BB-induced biological phenotype of VSMCs. (a) The mRNA expression of DLEU2 was analyzed using RT-qPCR in human atherosclerotic serum (n = 34) and normal serum (n = 20). (b) The mRNA expression of DLEU2 was analyzed using RT-qPCR in Control, PDGF-BB, PDGF-BB+pcDNA3.1-NC, PDGF-BB+DLEU2 and PDGF-BB+siDLEU2 groups. (c) Cell Counting Kit-8 (CCK-8) assay was performed to test the cell viability in each group. (d-e) The cell migration ability in each group was analyzed by wound healing assay. (f-g) Transwell assay was applied to determine cell invasion ability. β-actin was used as a control. PDGF-BB: platelet-derived growth factor-BB. RT-qPCR: reverse transcription quantitative PCR. NC: negative control. VSMCs: vascular smooth muscle cells. All experiments were repeated at least three times. **P < 0.01, ***P < 0.001 vs. Control, ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 vs. PDGF-BB+pcDNA3.1-NC. Data were expressed as mean ± standard deviation. Data between two groups or among multiple groups were analyzed by independent sample t test or one-way ANOVA, followed by Tukey’s post hoc test.