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. 2022 Sep 6;14(1):2115200. doi: 10.1080/19420862.2022.2115200

Figure 1.

Panel A: schematic representation of the three libraries, one with diversity in LCDR1 and 2, another with diversity in LCDR3, and the last with diversity in HCDR1 and 2. Panel B: surface plasmon resonance sensorgrams obtained from the parental antibody showing a dissociation constant of 7.4 nM. Panel C: An example of the construction of one of the libraries by assembling the LCDR1 collection and the LCDR2 collection with the remainder of the antibody.

(a) Schematic representation of the parental antibody scFv and the three libraries produced in phase 1: L1L2, L3, and H1H2. Each library has diversity introduced in the indicated CDRs. (b) Surface-plasmon resonance sensorgram of the parental antibody binding to the antigen at increasing concentrations (0.16 nM to 100 nM with a 5-fold stepwise increase). (c) Schematic representation of the L1L2 library assembly.